Genetic Linkage Map
IntroductionGenetic linkage map, constructed from a population segregation, has become a vital tool in different species for identifying quantitative trait locus (QTL) analysis, promoting the quality of genome assembly and assisting crop breeding . In recent years, the application of next generation sequencing (NGS) strategy made it possible to develop millions single nucleotide polymorphism (SNP) markers at once. Based on SNPs, a high-density genetic map with thousands of markers, even more, could be constructed for revealing the genetic basis of different interesting traits, such as disease resistance, growth habits, fruit quality, sex determination and so on.
Data analysis Pipeline
1. Highmap: a kind of software that was widely used for construction of linkage map was developed independently by Biomarker.
2. SLAF-seq: Specifc Length Amplifed Fragment sequencing, developed independently by Biomarker, is a efficient strategy for discovery of large scale SNP markers in a short time and and is a high-throughput, high accuracy and low cost method.
3. Analysis experience: we have constructed hundreds of linkage maps in recent years for nearly one hundred of species。
4. Publications: More than 100 linkage map articles were published using our method and the cumulative impact factor of all papers was mare than 360.
Biomarker Successful Cases
 Integrated analysis of phenome, genome, and transcriptome of hybrid rice uncovered multiple heterosis-related loci for yield increase. PNAS, 2016, IF=9.42
 TGW3, a Major QTL that Negatively Modulates Grain Length and Weight in Rice. Molecular plant, 2018, IF=8.83
 Genome sequencing of rice subspecies and genetic analysis of recombinant lines reveals regional yield- and quality associated loci. BMC Biology, 2018, IF=7.55
 STAYGREEN, STAY HEALTHY: a loss-of-susceptibility mutation in the STAYGREEN gene provides durable, broad-spectrum disease resistances for over 50 years of US cucumber production. New Phytologist, 2018, IF=7.43
 The R2R3 MYB transcription factor PavMYB10.1 involves in anthocyanin biosynthesis and determines fruit skin colour in sweet cherry (Prunus avium L.). Plant Biotechnology Journal, 2016, IF=6.09
 A re-sequencing-based ultra-dense geneticmap reveals a gummy stemblight resistance-associated gene in Cucumismelo. DNA Research, 2017, IF=5.40
 QTL mapping of downy and powdery mildew resistances in PI 197088 cucumber with genotyping-by-sequencing in RIL population。 Theoretical and Applied Genetic, 2017, IF=3。73
 High-resolution mapping of QTL for fatty acid composition in soybean using specific locus amplified fragment sequencing. Theoretical and Applied Genetic, 2017, IF=3.73
 The genetic architecture of growth traits in Salix matsudana under salt stress. Horticulture Research, 2017, IF=3.60
 A high density SLAF-SNP genetic map and QTL detection for fibre quality traits in Gossypium hirsutum。 BMC Genomics, 2018, IF=3。73